10 edition of Germline stem cells found in the catalog.
Includes bibliographical references and index.
|Statement||edited by Steven X. Hou, Shree Ram Singh.|
|Series||Methods in molecular biology -- 450, Methods in molecular biology (Clifton, N.J.) -- v. 450.|
|Contributions||Hou, Steven X., Singh, Shree Ram.|
|LC Classifications||QH588.S83 G47 2008|
|The Physical Object|
|Pagination||xii, 273 p. :|
|Number of Pages||273|
|ISBN 10||1603272135, 1603272143|
|ISBN 10||9781603272131, 9781603272148|
|LC Control Number||2007938052|
molecular distinction between somatic and germline stem cells is thought to be similarly blurry in other flatworms, such as schistosome’s free-living evolutionary cousin, the planarian. 19, Planarian germline stem cells resemble their somatic stem cells both in terms of morphology and. was not certified by peer review) is the author/funder. Stem cells are cells that can differentiate into other types of cells, and can also divide in self-renewal to produce more of the same type of stem cells.. Telomerase is active in normal stem cells.. In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts in early embryonic development, and adult stem cells.
Germline stem cells. Ed. by Steven X. Hou and Shree Ram Singh. Humana Press Inc. pages $ Hardcover. Differentiation of germline stem cells (GSCs) in the Drosophila ovary is induced by somatic escort cells (ECs), which extend membrane protrusions encapsulating the germline cells (GCs). Germline encapsulation requires activated epidermal growth factor receptor (Egfr) signaling within the ECs, following secretion of its ligands from the GCs.
Removal of DTCs by laser ablation causes germ cells to leave the mitotic cell cycle and enter meiosis (Kimble and White, ), and duplication or movement of DTCs promotes germline stem cells in the new DTC location (Feng et al., ; Kimble and White, ). Therefore, DTCs are both necessary and sufficient for germline proliferation during. Postnatal female germline stem cells (FGSCs) are a type of germline stem cell with self-renewal ability and the capacity of differentiation toward oocyte. The proliferation, differentiation, and apoptosis of FGSCs have been researched in recent years, but autophagy in FGSCs has not been explored. This study investigated the effects of the small-molecule compound 89 (C89) .
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Stem cells are a group of undifferentiated cells having the dual ability to self-renew and differentiate into functional mature cells. Somatic stem cells play essential roles in organogenesis and tissue maintenance, while germline stem cells (GSCs) can only produce gametes for reproduction (Li and Xie, ).In most of invertebrates and low vertebrates, both.
C. elegans germline stem cells are a particularly simple system for analysis of stem cell regulation. Their well-defined mesenchymal niche consists of a single cell, the Distal Tip Cell, which uses Notch signaling to maintain a pool of germline stem cells.
Downstream of Notch signaling a post-transcriptional regulatory network dictates self-renewal or by: This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells.
The Drosophila germline stem cells (GSCs) remain as one of the most well-understood adult stem cells. The number of stem cells that self-renews and differentiates must be tightly controlled to maintain tissue homeostasis.
The Drosophila GSCs are maintained by local signals emanated from the niche, which is composed of the surrounding somatic : Sharon Wui Sing Tan, Yu Cai, Gyeong Hun Baeg. Postnatal female germline stem cells (FGSCs) are a type of germline stem cell with self-renewal ability and the capacity of differentiation toward oocyte.
The proliferation, differentiation, and apoptosis of FGSCs have been researched in recent years, but autophagy in.
Following the first cleavage divisions, cells that receive the germ plasm are destined to become the germline stem cells. Such a pre-determination model of germ cell specification assures that germ cells are set-aside during the earliest steps of embryonic development, protecting them from the lineage specification and differentiation events.
Thus, several reports have documented the production of multi- or pluripotency germline stem cells in the mouse. In parallel to these studies of mice multipotent germline stem cells, several groups have reported a collection of genes and biomarkers that are non-exclusively expressed by mouse SSCs (Reviewed in Caires et al., ).
"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating germline stem cells. This book is written for basic science and clinical researchers as well as those interested in.
Stem cells are a group of undifferentiated cells having the dual ability to self-renew and differentiate into functional mature cells. Somatic stem cells play essential roles in organogenesis and tissue maintenance, while germline stem cells (GSCs) can only produce gametes for reproduction (Li and Xie, ).In most of invertebrates and low vertebrates, both Cited by: The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in.
By David A. Dansereau, Paul Lasko (auth.), Steven X. Hou, Shree Ram Singh (eds.) The wisdom received through learning germline stem cells, the resource of human and animal replica, may possibly locate quick software in retaining endangered flora and fauna, handling advertisement farm animals, overcoming fertility difficulties in people, and treating.
Germline stem cell homeostasis Jonathan O. Nelson, Cuie Chen and Yukiko Yamashita 7. Heterogeneous, dynamic, and stochastic nature of mammalian spermatogenic stem cells Shosei Yoshida 8.
Sperm success and immunity Stuart Wigby, Susan S. Suarez, Brian P. Lazzaro, Tomaso Pizzari and Mariana F. Wolfner 9. A symbiont’s guide to the germline. Stem cell self-renewal is controlled by concerted actions of extrinsic niche signals and intrinsic factors in a variety of systems.
Drosophila ovarian germline stem cells (GSCs) have been one of the most productive systems for identifying the factors controlling self-renewal. The differentiation factor BAM is necessary and sufficient for GSC. Buy Germline Stem Cells (Methods in Molecular Biology) on FREE SHIPPING on qualified orders Germline Stem Cells (Methods in Molecular Biology): Hou, Steven X., Singh, Shree Ram: : Books.
ISBN: OCLC Number: Description: xii, pages: illustrations. Contents: pt. Identification and regulation of germline stem cells in model organisms --The development of germline stem cells in Drosophila / David A. Dansereau and Paul Lasko --Analysis of the C. elegans germline stem cell region / Sarah.
Germline stem cells are key to genome transmission to future generations. Over recent years, there have been numerous insights into the regulatory mechanisms that govern both germ cell specification and the maintenance of the germline in adults.
Complex regulatory interactions with both the niche and the environment modulate germline stem cell function. Germline stem cells (GSCs) are a source of continuous production of gametes, sperms, and eggs, with the ability to produce a differentiated daughter cell and a daughter stem cell through asymmetric self-renewal cell division.
Molecular mechanisms governing stem cell maintenance and differentiation have been unearthed by studying the Drosophila ovary, in which eggs are. Stem cells are endowed with the unique ability to generate daughter cells and selfrenew.
In the adult gonads of many organisms, a specialized population of stem cells (termed germline stem cells; GSCs) is maintained to replenish the reservoir of germ cells whose numbers are depleted by gamete production.
As parent cells of gametes (oocytes and sperm), GSCs are also. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques.
Rats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species.
Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated. observed that stem cells from donor pup testes were more efficiently transduced (20% of cultured cells survived and were transduced) than male germline stem cells from crypt-orchid adult donors (only % of cultured cells survived and were transduced).
They also observed that wild-type recipients gave rise to wild-type progeny only and did not. Although males retain germline stem cells (GSCs) for spermatogenesis throughout adult life, oocyte production in females of most mammalian species is believed to cease before birth 1,2,3,4,5.The expansion of knowledge in the germline stem cell field is rapid and it is important that key developments are shared in a timely manner among the community members.
This conference will bring together leading scientists at the forefront of international research in the germ cell field in a range of vertebrate (primarily mammalian) systems.